Amino acid composition of Clostridium botulinum type A toxin
نویسندگان
چکیده
منابع مشابه
The elemental and amino acid composition of crystalline Clostridium botulinum type A toxin.
The isolation of Clostridium botulinurn type A toxin in crystalline form (1, 2) has led to an interest in its chemical composition. An apparently complete analysis of the pure crystalline toxin is presented in this paper. Preliminary studies have indicated that the toxin is a protein with an isoelectric point of pH 5.6, possessing the properties of a globulin (l-3). It is free from carbohydrate...
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introduction: widespread medical usage of botulinum toxins has increased the need for the precise analysis of its biological activity. the aim of this study was production and crystallization of the botulinum type a toxins. methods: in this study, clostridium botulinum was grown and inoculated into the tripticase soya broth culture media, under anaerobic condition and toxin production was monit...
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Botulism is a serious paralytic disease caused by Clostridium botulinum toxin in foods. There are seven recognized serotypes of botulinum neurotoxins among which the principal prevalent types in humans include A, B and E. Infant botulism results from intestinal colonization and toxin production by C. botulinum spores in babies less than 1 year old. Honey is the most important food discriminated...
متن کاملRadioimmunoassay for type A toxin of Clostridium botulinum.
A preparation of pure type A toxin of Clostridium botulinum was labeled with (131)I in the presence of chloramine-T and carrier-free isotope. The radioactive toxin ((131)I Tox) was used in a radioimmunoassay procedure similar to that of Berson and Yalow. Dilutions of antibody to the toxin, capable of binding 50% of the added (131)I Tox, were mixed with a sample of the labeled toxin and various ...
متن کاملPhysicochemical Properties of Crystalline Clostridium Botulinum Type a Toxin*
Interest in the chemical nature and mode of action of bacterial toxins has been renewed by the simultaneously announced crystallization of botulinal (1) and tetanal toxins (2) and the recent purification of several toxoids (3-5). Of these, crystalline Clostridium botulinurn type A toxin alone has been submitted to an apparently complete elementary and amino acid analysis (6), as well as to prel...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1966
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.92.3.796-797.1966